More than 70 % chicory inulin and 85 % asparagus inulin were hydrolyzed under optimized parameters at 60 ☌. Raw inulin from chicory and asparagus was extracted and supplied in 17.5 % for enzymatic hydrolysis as a replacement of pure inulin. Immobilized enzyme was reused for ten cycles. At high substrate loading (17.5 % inulin), using 9.9 U/g immobilized inulinase at 60 ☌ in 12 h, maximum sugar yield was 171.1 ± 0.3 mg/ml and productivity was 14.25 g/l/h. Statistical model was proposed with an objective of optimizing enzymatic inulin hydrolytic process. Immobilized biocatalyst displayed significant increase in thermostability at 60 and 65 ☌. Fourier transform infrared spectroscopy (FTIR) analysis confirmed linkage of inulinase with chitosan particles. Inulinase was immobilized on chitosan particles and employed for fructose production by inulin hydrolysis. In order to meet the demand of concentrated fructose syrup, inulin hydrolysis at high substrate loading is essential.
For commercial production, use of immobilized inulinase is advantageous as it offers reutilization of enzyme and increase in stability. Biocatalytic production of fructose from inulin employing inulinase is the most promising alternative for fructose production. Fructose, a monosaccharide, has gained wide applications in food, pharmaceutical and medical industries because of its favourable properties and health benefits.
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